Part:BBa_K2360012
pChrB-ChrB+SRRz+rrnB terminater
It is a high-efficiency chromium detecting device. This device consist two parts, the lysis module (BBa_K2360000) and chromium detecting module(BBa_K2360007).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the chromium ion. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 136
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 911
- 1000COMPATIBLE WITH RFC[1000]
Figure 1. Bio-circuit of BBa_K2360012
Experiment
Lysis gene expression
We measured the growth curve of our engineering bacteria in different concentration of nickel ions. It was a pity that we found the OD600 of E. coli with did not decline after we added Cr6+, which showed this device did not work as we expected.
Figure 2. the growth curve of chromium detecting device in different concentration of chromium ions.
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